Methods in Experimental Pathology
Institute of Pathology, College of Medicine
National Taiwan University
Second semester, 2001 (February 20 through June 18, 2002)
Time: 10:00 A.M.-12:00 P.M., Thursday
Place: New Building, 3rd Floor, Department of Pathology, Conference Room (Rm 3-80) National Taiwan University Hospital
(台大醫院新大樓三樓病理部討論室3-80)
Date Title Speaker
2/21 Introduction, polyclonal and monoclonal antibodies林欽塘
2/28 Cell and tissue cultures (primary culture) 林欽塘
3/07 Immunohistochemistry and immunoelectron microscopy林欽塘
3/14 Protein purification and identification 吳漢忠
3/21 PCR, RT-PCR, immuno-PCR, and Laser capture microdissection 吳漢忠
3/28 In situ nucleic acid hybridization, in situ PCR hybridization, in situ RT-PCR hybridization 林欽塘
4/11 (8:10-10:00am) Cytogenetic analysis and cytology田蕙芬,彭曄
4/18 Midterm examination (09:30-12:00)
4/25 Functional analysis of gene product: sense, antisense and S-oligonucleotide transfections, and antibody microinjection.吳漢忠
5/02 Applications of DNA chips in Biomedical studies 陳建尉
5/09 Using molecular approach to study the critical genes involved in spermatogensis 唐堂
5/16 Trangenic mice and gene targeting 林淑華
5/23 Evaluation of oxidative stress by FACScan, QT-PCR and chemiluminescencer 鄭劍廷
5/30 Application of phage display 吳漢忠
6/06 DNA transfections and reporter gene analysis.Method to measure transcriptional regulation 吳國瑞
6/13 Final examination (Thu. 9:00-12:00)